Sections:

Physical Mapping, Microarrays:

Sergey Kalachikov, Eftihia Cayanis, Anthi Georghiou, Stuart G. Fischer, Samantha H. Park, Elita Teplitskaya.

Columbia Genome Center

Pieter De Jong, Baohui Zhao

BacPac Resources Laboratory

- use of largely STS-based hybridization and PCR methods for assembling BAC and other clone libraries; verification that BAC's used in subsequent shotgun sequencing are appropriate (largely non-redundant with previously shotgunned BAC's, and lacking substantial deletions or rearrangements); confirmation of the map using pulsed-field gels and Southern analysis; microarray expression studies and microarray studies for comparison of genomes of different Legionella species.

High-Throughput Sequencing:

James J. Russo, Minchen Chien, Shundi Shi, Jing Chen, Gifty Asamani, Kendra Hill, John Nuara, Marc Feder, Justin Rineer, Joseph J.Greenberg, Valeria Steshenko, John R. Edwards, I-Chun Chou, William Iannuccilli, Michael E. Ulz, Dae H. Kim, Curtis Goldsberry, Xiaoyan Qu.

Columbia Genome Center

- shotgun sequencing from chromosomal and BAC DNA; designing finishing reactions; sequence editing.

Informatics:

Irina Morozova, Huitao Sheng, Shawn M. Gomez, Pavel Morozov, Andrey Rzhetsky, Peisen Zhang
Columbia Genome Center

- maintenance of Legionella database, design of programs to assist in mapping, sequence assembly and annotation; metabolic pathway prediction and compative analysis of Legionella and other intracellular pathogens.

Functional Analysis:

Howard A. Shuman, Sergey Pampou, Gil Segal

Dept. of Microbiology, Columbia Genome Center

- use of allelic exchange to delete putative genes in Legionella to assess their role in bacterial life cycle, infectivity and cell killing; comparison of genes among variant strains; comparison of expressed gene and protein repertoire in various stages of bacterial life cycle.